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Preliminary Program


 

- COURSE SCHEDULE -

 

 

 

 

 

Monday
(lecture room, cloakroom and refreshments: auditorium on the ground floor, No. 0.195)

 

8:30 – 9:00

Registration

 

9:00 – 9:10

Introduction/layout of the course

Pavel Hozák

9:10 – 9:30

Short presentations of the participants

 

9:30 – 10:15

Sample preparation for transmission electron microscopy – Lecture 1

Andreas Nowak

10:15 – 10:30

Coffee break

 

10:30 – 11:15

Cryoelectron microscopy – sample preparation techniques 

Andreas Nowak

11:15 – 13:30

Practical sessions in two groups
- Tokuyasu technique - group A
- HPF and AFS – group B

Andreas Nowak
Frederic Lerough

13:30 – 14:00

Lunch

 

14:00 – 16:15

Practical sessions in two groups
- Tokuyasu technique - group A
- HPF and AFS – group B

Andreas Nowak
Frederic Lerough

16:15 – 17:00

Theory: How to choose a microscope for your applications?

Gaurav Sharma

 

Tuesday
(cloakroom and refreshments: glass seminar room on the ground floor)

 

09:00 – 09:50

Basics of TEM (the lecture takes place on the 2nd floor in seminar room)

  1. Theory: General TEM construction
  2. Alignments (recognizing mis-alignments), resolution, contrast
  3. Energy filtered TEM
  4. Digital cameras

Daniel Nėmeček

09:50 – 10:00

Coffee break

 

10:00 – 13:00

Practical session: Alignments
- Morgagni (room 01.152) – group 1
- CM 100 (building "U") – group 2
- T20 (room 01.150) – group 3
Direct alignments, alignments procedures, test specimens

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

13:00 – 14:00

Lunch

 

14:00 – 17:00

Practical session: TEM imaging
- Morgagni (room 01.152) – group 3
- CM 100 (building "U") – group 1
- T20 (room 01.150) – group 2
Bright/dark field, resolution, focus, astigmatism, contrast

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

19:00 – ?

Evening dinner

 

 

Wednesday
(cloakroom and refreshments: glass seminar room on the ground floor)

 

09:00 – 12:00

Practical session
- Morgagni (room 01.152) – group 2
- CM 100 (building "U") – group 3
- T20 (room 01.150) – group 1

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

12:00 – 13:00

Lunch

 

13:00 – 13:50

Theory: High-resolution cryo electron microscopy

  1. Sample vitrification
  2. Low-dose imaging
  3. Image processing and single particle analysis

(the lecture takes place on the 3rd floor in seminar room)

Daniel Nėmeček

13:50 – 14:00

Coffee break

 

14:00 – 17:00

Practical session
- Morgagni (room 01.152) – group 1
- CM 100 (building "U") – group 2
- T20 (room 01.150) – group 3

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

 

Thursday
(lecture room, cloakroom and refreshments: auditorium on the ground floor, No. 0.195)

 

09:00 – 12:00

Practical session: Repetition of different parts
- Morgagni (room 01.152) – group 3
- CM 100 (building "U") – group 1
- T20 (room 01.150) – group 2

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

12:00 – 13:00

Lunch

 

13:00 – 13:50

Theory: The best resolution and instrument requirements 
The highest resolution in Life Sciences, best practice in biology

Gaurav Sharma

13:50 – 14:05

Short test

 

14:05 – 14:15

Coffee break

 

14:15 – 17:15

Practical session (continued)
- Morgagni (room 01.152) – group 2
- CM 100 (building "U") – group 3
- T20 (room 01.150) – group 1

Gaurav Sharma
Oldøich Benada
Daniel Nėmeček

 

Friday
(lecture room, cloakroom and refreshments: auditorium on the ground floor, No. 0.195)

 

09:00 – 11:00

- Cryo-TEM – demo of sample preparation and imaging basics
(room 01.150, group A)
- Theory: Cryo-TEM
(auditorium on the ground floor, No. 0.195, group B)

Gaurav Sharma
/
Daniel Nėmeček

11:00 – 12:00

Lunch

 

12:00 – 14:00

- Cryo-TEM – demo of sample preparation and imaging basics
(room 01.150, group A)
- Theory: Cryo TEM Tomography
(auditorium on the ground floor, No. 0.195, group B)
1. Cryo electron tomography workflow
2. Large volume analysis
3. 3D tomography reconstruction
4. Sub-tomogram averaging
5. Questions and discussion to all topics
 

Gaurav Sharma
/
Daniel Nėmeček

14:00 – 14:20

Closure of the training part, results of the test

Pavel Hozák

14:20 – 15:00

Evaluation of the workshop

Pavel Hozák