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Preliminary Program


 

- COURSE SCHEDULE -

 

 

 

 

 

Monday 26th November
(lecture room, cloakroom and refreshments: auditorium on the ground floor, No. 0.195)

 

8:30 – 9:00

Registration

 

9:00 – 9:15

Introduction/layout of the course

Pavel Hozák

9:15 – 9:45

Short presentations of the participants

 Participants

9:45 – 10:30

Sample preparation for transmission electron microscopy

Andreas Nowak

10:30 – 10:45

Coffee break

 

10:45 – 11:15

Cryo sample preparation techniques for TEM / cryo TEM

Andreas Nowak

11:15 – 13:30

Practical sessions in two groups

  • Tokuyasu technique - group yellow
  • HPF and AFS – group purple

 

Andreas Nowak
Frederic Lerough

13:30 – 14:00

Lunch

 

14:00 – 16:15

Practical sessions in two groups

  • Tokuyasu technique – group purple
  • HPF and AFS – group yellow

 

Andreas Nowak
Frederic Lerough

16:15 – 17:00

Q&A / application questions concerning entire sample prep techniques 

Frederic Leroux/ ALL

 

Tuesday

 

09:00 – 09:50

Basics of TEM 

  • General TEM construction
  • Alignments (recognizing mis-alignments), resolution, contrast
  • Energy filtered TEM
  • Digital cameras

Daniel Nėmeček

09:50 – 10:00

Coffee break

 

10:00 – 13:00

Practical session: Alignments
- Morgagni (room 01.152) – group 1
- CM 100 (building "U") – group 2
- T20 (room 01.150) – group 3
Direct alignments, alignments procedures, test specimens

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

13:00 – 14:00

Lunch

 

14:00 – 17:00

Practical session: TEM imaging
- Morgagni (room 01.152) – group 3
- CM 100 (building "U") – group 1
- T20 (room 01.150) – group 2
Bright/dark field, resolution, focus, astigmatism, contrast

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

 

Wednesday

 

09:00 – 12:00

Practical session
- Morgagni (room 01.152) – group 2
- CM 100 (building "U") – group 3
- T20 (room 01.150) – group 1

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

12:00 – 13:00

Lunch

 

13:00 – 13:50

Theory: How to choose a microscope for your applications?

Gaurav Sharma

13:50 – 14:00

Coffee break

 

14:00 – 17:00

Practical session
- Morgagni (room 01.152) – group 1
- CM 100 (building "U") – group 2
- T20 (room 01.150) – group 3

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

 

Thursday

 

09:00 – 12:00

Practical session: Repetition of different parts
- Morgagni (room 01.152) – group 3
- CM 100 (building "U") – group 1
- T20 (room 01.150) – group 2

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

12:00 – 13:00

Lunch

 

13:00 – 13:50

Theory: The best resolution and instrument requirements 
The highest resolution in Life Sciences, best practice in biology

Gaurav Sharma

13:50 – 14:05

Short test

 

14:05 – 14:15

Coffee break

 

14:15 – 17:15

Practical session (continued)
- Morgagni (room 01.152) – group 2
- CM 100 (building "U") – group 3
- T20 (room 01.150) – group 1

Gaurav Sharma Oldøich Benada
Daniel Nėmeček

 

Friday
(lecture room, cloakroom and refreshments: auditorium on the ground floor, No. 0.195)

 

09:00 – 11:00

- Cryo-TEM – demo of sample preparation and imaging basics (room 01.150, group yellow)
- Theory: Cryo-TEM – (lecture room, group purple)

Gaurav Sharma/Daniel Nėmeček

11:00 – 12:00

Lunch

 

12:00 – 14:00

- Cryo-TEM – demo of sample preparation and imaging basics (room 01.150, group purple)
- Theory: Cryo TEM (lecture room, group yellow)

  • Sample vitrification
  • Low-dose imaging
  • Image processing and single particle analysis
  • Cryo electron tomography workflow
  • Large volume analysis
  • 3D tomography reconstruction
  • Sub-tomogram averaging
  • Questions and discussion to all topics

Gaurav Sharma/Daniel Nėmeček

14:00 – 14:20

Closure of the training part, results of the test

Pavel Hozák

14:20 – 15:00

Evaluation of the workshop

Pavel Hozák